Human DR1 Recombinant Protein (RPPB3375)

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SKU:
RPPB3375
Product type:
Recombinant Protein
Size:
25ug
Species:
Human
Target:
DR1
Synonyms:
NC2
Synonyms:
NC2-BETA
Synonyms:
Negative cofactor 2-beta
Source:
Escherichia Coli
Uniprot:
Q01658
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Description

Product Name:Human DR1 Recombinant Protein
Product Code:RPPB3375
Size:25µg
Species:Human
Target:DR1
Synonyms:NC2, NC2-BETA, Negative cofactor 2-beta.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:DR1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 0.1mM PMSF, 0.1M NaCl & 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI SSEANEICNK SEKKTISPEH VIQALESLGF GSYISEVKEV LQECKTVALK RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ AQLAAASASA NQAGSSQDE EDDDDI

DR1 is a phosphoprotein that inhibits both basal and activated levels of transcription. DR1 is phosphorylated in vivo which affects its interaction with TBP. DR1 has a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 alters the physiological balance of transcriptional output in favor of polymerase I.

DR1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (1-176 a.a.) and having a molecular mass of 21.6 kDa. The DR1 is fused to 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:DR1: The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. Heterodimer with DRAP1. DR1 exists in solution as a homotetramer that dissociates during interaction with TBP and then, after complexing with TBP, reassociates at a slow rate, to reconstitute the tetramer. Interacts with NFIL3. Component of the ADA2A-containing complex (ATAC), composed of CSRP2BP, KAT2A, TADA2L, TADA3L, ZZ3, MBIP, WDR5, YEATS2, CCDC101 and DR1. Belongs to the NC2 beta/DR1 family.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: nucleus

Molecular Function:protein binding; TATA-binding protein binding; transcription corepressor activity; transcription factor binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene encodes a TBP- (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The encoded protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. The binding of DR1 repressor complexes to TBP-promoter complexes may establish a mechanism in which an altered DNA conformation, together with the formation of higher order complexes, inhibits the assembly of the preinitiation complex and controls the rate of RNA polymerase II transcription. [provided by RefSeq, Jul 2008]
UniProt Code:Q01658
NCBI GenInfo Identifier:401162
NCBI Gene ID:1810
NCBI Accession:Q01658.1
UniProt Related Accession:Q01658
Molecular Weight:19,444 Da
NCBI Full Name:Protein Dr1
NCBI Synonym Full Names:down-regulator of transcription 1
NCBI Official Symbol:DR1  
NCBI Official Synonym Symbols:NC2; NC2B; NC2-BETA  
NCBI Protein Information:protein Dr1
UniProt Protein Name:Protein Dr1
UniProt Synonym Protein Names:Down-regulator of transcription 1; Negative cofactor 2-beta; NC2-beta; TATA-binding protein-associated phosphoprotein
Protein Family:Dr1-associated corepressor
UniProt Gene Name:DR1  
UniProt Entry Name:NC2B_HUMAN
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