The Human DPD (Deoxypyridinoline) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of DPD levels in human serum, plasma, and urine samples. This kit provides accurate and reproducible results, making it perfect for a variety of research applications.DPD is a biomarker for bone resorption and is used to assess bone metabolism in conditions such as osteoporosis and Paget's disease.
Monitoring DPD levels can help researchers and healthcare professionals better understand bone health and disease progression, leading to improved diagnostic and treatment strategies.With its reliable performance and comprehensive capabilities, the Human DPD ELISA Kit is a valuable tool for studying bone metabolism and related disorders, ultimately advancing our knowledge and therapeutic approaches in the field.
Product Name:
Human DPD (Deoxypyridinoline) ELISA Kit
SKU:
HUES02949
Target:
Human DPD (Deoxypyridinoline)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DPD. During the reaction, DPD in the sample or standard competes with a fixed amount of DPD on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DPD. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DPD in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
90-105
94-107
Average (%)
92
96
101
1:4
Range (%)
91-105
92-103
97-113
Average (%)
96
98
104
1:8
Range (%)
89-102
92-106
93-106
Average (%)
95
97
100
1:16
Range (%)
90-105
89-100
98-115
Average (%)
97
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
93-106
99
Cell culture media (n=5)
87-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.7
29.2
74.7
9.7
31.8
73.5
Standard deviation
0.5
1.2
3.6
0.6
1.9
2.6
C V (%)
5.15
4.11
4.82
6.19
5.97
3.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of DPD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes DPD in samples. No significant cross-reactivity or interference between DPD and analogues was observed.
