SLC6A3,DAT1,Sodium-dependent dopamine transporter,Dopamine Active Transporter,SLC6-A3,Solute Carrier Family 6(neurotransmitter Transporter,Dopamine),Member 3
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Human Dopamine Transporter (DAT) ELISA Kit is a highly reliable and sensitive tool for the quantitative detection of dopamine transporter levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is specifically designed to provide accurate and reproducible results, making it ideal for a wide range of research applications and studies.The dopamine transporter is a crucial protein involved in the reuptake of dopamine, a neurotransmitter that plays a key role in various physiological and neurological processes. Dysregulation of dopamine transporter activity has been linked to several neuropsychiatric disorders, including Parkinson's disease, schizophrenia, and addiction.
Therefore, the measurement of dopamine transporter levels can provide valuable insights into the pathophysiology of these disorders and aid in the development of potential therapeutic strategies.By utilizing the Human DAT ELISA Kit, researchers can effectively study the role of dopamine transporter in health and disease, advancing our understanding of neurobiology and potentially leading to the discovery of novel treatment approaches. Trust in the accuracy and precision of this kit to support your research endeavors and contribute to scientific advancements in the field of neuroscience.
Product Name:
Human Dopamine Transporter (DAT) ELISA Kit
Product Code:
HUDL00790
Size:
96 Assays
Target:
DAT
Synonyms:
SLC6A3, DAT1, Sodium-dependent dopamine transporter, Dopamine Active Transporter, SLC6-A3, Solute Carrier Family 6(neurotransmitter Transporter,Dopamine),Member 3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
