Human Dopa Decarboxylase Recombinant Protein (RPPB1583)
- SKU:
- RPPB1583
- Product type:
- Recombinant Protein
- Size:
- 20ug
- Species:
- Human
- Target:
- Dopa Decarboxylase
- Synonyms:
- DDC
- AADC
- Aromatic-L-amino-acid decarboxylase
- DOPA decarboxylase
- Source:
- Escherichia Coli
- Uniprot:
- P20711
Description
| Product Name: | Human Dopa Decarboxylase Recombinant Protein |
| Product Code: | RPPB1583 |
| Size: | 20µg |
| Species: | Human |
| Target: | Dopa Decarboxylase |
| Synonyms: | DDC, AADC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | The Dopa decarboxylase protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8, 2mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH TRSMNASEFR RRGKEMVDYV ANYMEGIEGR QVYPDVEPGY LRPLIPAAAP QEPDTFEDII NDVEKIIMPG VTHWHSPYFF AYFPTASSYP AMLADMLCGA IGCIGFSWAA SPACTELETV MMDWLGKMLE LPKAFLNEKA GEGGGVIQGS ASEATLVALL AARTKVIHRL QAASPELTQA AIMEKLVAYS SDQAHSSVER AGLIGGVKLK AIPSDGNFAM RASALQEALE RDKAAGLIPF FMVATLGTTT CCSFDNLLEV GPICNKEDIW LHVDAAYAGS AFICPEFRHL LNGVEFADSF NFNPHKWLLV NFDCSAMWVK KRTDLTGAFR LDPTYLKHSH QDSGLITDYR HWQIPLGRRF RSLKMWFVFR MYGVKGLQAY IRKHVQLSHE FESLVRQDPR FEICVEVILG LVCFRLKGSN KVNEALLQRI NSAKKIHLVP CHLRDKFVLR FAICSRTVES AHVQRAWEHI KELAADVLRA ERE |
Dopa decarboxylase is a homodimeric, pyridoxal phosphate dependent enzyme. Dopa decarboxylase is involved in 2 metabolic pathways, synthesizing 2 significant neurotransmitters the take part in numerous clinical disorders, including Parkinson’s disease. Dopa decarboxylase is located in different areas of the brain and is mostly found in basal ganglia. Dopa decarboxylase catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopa, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in Dopa decarboxylase leads to aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that causes combined serotonin and catecholamine deficiency.
Dopa decarboxylase human recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 503 amino acids (1-480 a.a.) and having a molecular mass of 56.4 kDa. The Dopa decarboxylase is fused to a 23 amino acid His Tag at N-terminus and purified by conventional chromatpgraphy.
| UniProt Protein Function: | DDC: Catalyzes the decarboxylation of L-3,4- dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Homodimer. Belongs to the group II decarboxylase family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - tryptophan; EC 4.1.1.28; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; Nuclear receptor co-regulator; Amino Acid Metabolism - phenylalanine; Lyase Chromosomal Location of Human Ortholog: 7p12.2 Cellular Component: synaptic vesicle; cell soma; axon; cytosol Molecular Function:aromatic-L-amino-acid decarboxylase activity; protein domain specific binding; amino acid binding; protein binding; enzyme binding; pyridoxal phosphate binding Biological Process: circadian rhythm; amino acid metabolic process; catecholamine biosynthetic process; indolalkylamine biosynthetic process; multicellular organismal aging; isoquinoline alkaloid metabolic process; dopamine biosynthetic process; serotonin biosynthetic process; phytoalexin metabolic process; synaptic vesicle amine transport; response to pyrethroid Disease: Aromatic L-amino Acid Decarboxylase Deficiency |
| NCBI Summary: | The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011] |
| UniProt Code: | P20711 |
| NCBI GenInfo Identifier: | 311033369 |
| NCBI Gene ID: | 1644 |
| NCBI Accession: | P20711.2 |
| UniProt Secondary Accession: | P20711,O88533, P14173, |
| UniProt Related Accession: | P20711 |
| Molecular Weight: | 480 |
| NCBI Full Name: | Aromatic-L-amino-acid decarboxylase |
| NCBI Synonym Full Names: | dopa decarboxylase (aromatic L-amino acid decarboxylase) |
| NCBI Official Symbol: | DDC |
| NCBI Official Synonym Symbols: | AADC |
| NCBI Protein Information: | aromatic-L-amino-acid decarboxylase |
| UniProt Protein Name: | Aromatic-L-amino-acid decarboxylase |
| UniProt Synonym Protein Names: | DOPA decarboxylase; DDC |
| Protein Family: | Aromatic-L-amino-acid decarboxylase |
| UniProt Gene Name: | DDC |
| UniProt Entry Name: | DDC_HUMAN |
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