The Human DNMT1 (DNA Methyltransferase 1) ELISA Kit is specifically designed for the quantitative detection of DNMT1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for a variety of research applications.DNMT1 is a key enzyme involved in DNA methylation, playing a crucial role in gene regulation and genome stability. Dysregulation of DNMT1 has been linked to various diseases including cancer, genetic disorders, and developmental abnormalities.
Therefore, measuring DNMT1 levels can provide valuable insights into disease progression and treatment strategies.With its reliable performance and ease of use, the Human DNMT1 ELISA Kit from Assay Genie is an essential tool for researchers studying epigenetics, gene expression, and disease mechanisms. Invest in this kit to advance your research and uncover new discoveries in the field of molecular biology.
Product Name:
Human DNMT1 (DNA Methyltransferase 1) ELISA Kit
SKU:
HUES03427
Target:
Human DNMT1 (DNA Methyltransferase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DNMT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DNMT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DNMT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DNMT1. You can calculate the concentration of Human DNMT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-115
97-111
98-113
Average (%)
105
104
106
1:4
Range (%)
86-98
86-99
85-98
Average (%)
92
91
91
1:8
Range (%)
87-98
85-96
85-96
Average (%)
93
91
90
1:16
Range (%)
88-100
80-94
86-97
Average (%)
95
86
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-104
98
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
0.97
3.71
0.45
0.9
3.75
Standard deviation
0.03
0.05
0.13
0.03
0.04
0.2
C V (%)
6.52
5.15
3.5
6.67
4.44
5.33
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DNMT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DNMT1 in samples. No significant cross-reactivity or interference between Human DNMT1 and analogues was observed.
