The Human Discs Large Homolog 3 (DLG3) ELISA Kit is a reliable and accurate tool for the quantitative detection of DLG3 levels in human serum, plasma, and cell culture supernatants. DLG3 is a key protein involved in synaptic function and cell adhesion, playing a critical role in neurological disorders such as autism and schizophrenia.This ELISA kit offers high sensitivity and specificity, allowing for precise results that can be used in a variety of research applications.
By measuring DLG3 levels, researchers can gain valuable insights into the mechanisms underlying neurological conditions and potentially identify novel therapeutic targets.Overall, the Human DLG3 ELISA Kit from AssayGenie is a valuable resource for researchers seeking to better understand the role of DLG3 in neurological disorders and advance our knowledge of synaptic function and cell adhesion.
Product Name:
Human Discs, Large Homolog 3 (DLG3) ELISA Kit
Product Code:
HUDL00837
Size:
96 Assays
Target:
DLG3
Synonyms:
MRX, XLMR, MRX90, NE-Dlg, NEDLG, SAP102, Neuroendocrine-Dlg, Synapse-associated protein 102
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
