Human DHRS9 Recombinant Protein (RPPB1575)

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SKU:
RPPB1575
Product type:
Recombinant Protein
Size:
10ug
Species:
Human
Target:
DHRS9
Synonyms:
Dehydrogenase/reductase SDR family member 9
Synonyms:
3-alpha hydroxysteroid dehydrogenase
Synonyms:
3-alpha-HSD
Synonyms:
NADP-dependent retinol dehydrogenase/reductase
Source:
Escherichia Coli
Uniprot:
Q9BPW9
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Description

Product Name:Human DHRS9 Recombinant Protein
Product Code:RPPB1575
Size:10µg
Species:Human
Target:DHRS9
Synonyms:Dehydrogenase/reductase SDR family member 9, 3-alpha hydroxysteroid dehydrogenase, 3-alpha-HSD, NADP-dependent retinol dehydrogenase/reductase, RDH-E2, RDHL, Short-chain dehydrogenase/reductase retSDR8, DHRS9, RDH15, RDHTBE, SDR9C4, RETSDR8, 3ALPHA-HSD.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The DHRS9 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl, 1mM DTT and 0.1mM PMSF.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMRKGKL KIEDITDKYI FITGCDSGFG NLAARTFDKK GFHVIAACLT ESGSTALKAE TSERLRTVLL DVTDPENVKR TAQWVKNQVG EKGLWGLINN AGVPGVLAPT DWLTLEDYRE PIEVNLFGLI SVTLNMLPLV KKAQGRVINV SSVGGRLAIV GGGYTPSKYA VEGFNDSLRR DMKAFGVHVS CIEPGLFKTN LADPVKVIEK KLAIWEQLSP DIKQQYGEGY IEKSLDKLKG NKSYVNMDLS PVVECMDHAL TSLFPKTHYA AGKDAKIFWI PLSHMPAALQ DFLLLKQKAE LANPKAV

Dehydrogenase/reductase SDR family member 9 (DHRS9) functions as a homotetramer, which converts both 3-alpha-tetrahydroprogesterone (allopregnanolone) and 3-alpha-androstanediol to dihydroxyprogesterone and is believed to have a part in retinoic acid biosynthesis.

DHRS9 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 327 amino acids (18-319) and having a molecular mass of 35.9kDa.DHRS9 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:DHRS9: 3-alpha-hydroxysteroid dehydrogenase that converts 3- alpha-tetrahydroprogesterone (allopregnanolone) to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone. May play a role in the biosynthesis of retinoic acid from retinaldehyde, but seems to have low activity with retinoids. Can utilize both NADH and NADPH. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Cofactor and Vitamin Metabolism - retinol; Endoplasmic reticulum; Secreted; EC 1.1.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane

Molecular Function:alcohol dehydrogenase activity; racemase and epimerase activity; 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; retinol dehydrogenase activity

Biological Process: epithelial cell differentiation; retinol metabolic process; androgen metabolic process; 9-cis-retinoic acid biosynthetic process; progesterone metabolic process

NCBI Summary:This gene encodes a member of the short-chain dehydrogenases/reductases (SDR) family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. This protein demonstrates oxidoreductase activity toward hydroxysteroids and is able to convert 3-alpha-tetrahydroprogesterone to dihydroxyprogesterone and 3-alpha-androstanediol to dihydroxyprogesterone in the cytoplasm, and may additionally function as a transcriptional repressor in the nucleus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:Q9BPW9
NCBI GenInfo Identifier:74752227
NCBI Gene ID:10170
NCBI Accession:Q9BPW9.1
UniProt Secondary Accession:Q9BPW9,Q5RKX1, Q9NRA9, Q9NRB0, B7Z416, D3DPC1,
UniProt Related Accession:Q9BPW9
Molecular Weight:319
NCBI Full Name:Dehydrogenase/reductase SDR family member 9
NCBI Synonym Full Names:dehydrogenase/reductase (SDR family) member 9
NCBI Official Symbol:DHRS9  
NCBI Official Synonym Symbols:RDHL; RDH15; RDH-E2; RDHTBE; SDR9C4; RDH-TBE; RETSDR8; 3ALPHA-HSD; 3-alpha-HSD  
NCBI Protein Information:dehydrogenase/reductase SDR family member 9; retinol dehydrogenase homolog; 3-alpha hydroxysteroid dehydrogenase; short-chain dehydrogenase/reductase retSDR8; NADP-dependent retinol dehydrogenase/reductase; short chain dehydrogenase/reductase family 9C, member 4; tracheobronchial epithelial cell-specific retinol dehydrogenase
UniProt Protein Name:Dehydrogenase/reductase SDR family member 9
UniProt Synonym Protein Names:3-alpha hydroxysteroid dehydrogenase; 3-alpha-HSD; NADP-dependent retinol dehydrogenase/reductase; RDH-E2; RDHL; Short-chain dehydrogenase/reductase retSDR8; Tracheobronchial epithelial cell-specific retinol dehydrogenase; RDH-TBE
Protein Family:Dehydrogenase/reductase SDR family
UniProt Gene Name:DHRS9  
UniProt Entry Name:DHRS9_HUMAN
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