Human Deoxyribonuclease I Like Protein 3 ELISA Kit
The Human Deoxyribonuclease I-like Protein 3 (DNaseIL3) ELISA Kit is specifically designed for the accurate detection of DNaseIL3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.DNaseIL3 is an essential protein involved in DNA degradation and repair processes, playing a critical role in maintaining genomic stability. Dysregulation of DNaseIL3 expression has been linked to various diseases, including cancer, autoimmune disorders, and inflammatory conditions.
Therefore, this ELISA kit serves as a valuable tool for studying the role of DNaseIL3 in disease pathogenesis and potentially identifying new therapeutic targets.With its user-friendly protocol and short assay time, the Human DNaseIL3 ELISA Kit is the ideal choice for researchers seeking accurate and reproducible measurements of DNaseIL3 levels in biological samples. Order now to advance your research in the field of DNA biology and disease mechanisms.
Product Name:
Human Deoxyribonuclease I Like Protein 3 ELISA Kit
Product Code:
HUDL00850
Size:
96 Assays
Target:
DNASE1L3
Synonyms:
Liver and spleen DNase(LS-DNase, LSD), DNase I homolog protein DHP2, Deoxyribonuclease I-like 3(DNase I-like 3)
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
