Human Delta-Sleep Inducing Peptide (dSIP) ELISA Kit
The Human Delta Sleep-Inducing Peptide (DSIP) ELISA Kit is a reliable tool for detecting levels of DSIP in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results for a variety of research applications.DSIP is a peptide known for its role in promoting deep sleep and influencing the body's circadian rhythm. Research has shown that DSIP levels can be altered in conditions such as insomnia, depression, and stress, making it a valuable biomarker for studying these disorders and potential therapeutic interventions.
This ELISA kit is a valuable resource for researchers studying sleep disorders, mental health conditions, and stress-related diseases, providing essential insights into the role of DSIP in regulating sleep patterns and overall well-being.
Product Name:
Human Delta-Sleep Inducing Peptide (dSIP) ELISA Kit
Product Code:
HUDL00881
Size:
96 Assays
Target:
dSIP
Synonyms:
DSIPI, TSC22D3, DIP, GILZ, TSC-22R, hDIP, TSC22 Domain Family Protein 3, Glucocorticoid-Induced Leucine Zipper, Delta Sleep Inducing Peptide,Immunoreactor
Detection Method:
Sandwich
Range:
125-8,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
