Human Deleted In Malignant Brain Tumors 1 (DMBT1) ELISA Kit

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SKU:
HUDL00843
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9UGM3
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GP340,SAG,Muclin,Glycoprotein 340,Hensin,Salivary agglutinin,Surfactant pulmonary-associated D-binding protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Lung Lavage Fluid And Other Biological Fluids
Frequently bought together:

Description

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Human Deleted In Malignant Brain Tumors 1 (DMBT1) ELISA Kit

The Human Deleted in Malignant Brain Tumors 1 (DMBT1) ELISA Kit is specially designed for the precise detection of DMBT1 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for a variety of research applications.DMBT1 is a protein that is associated with various cancers, including brain tumors, as well as inflammatory and immune-related diseases.

By measuring DMBT1 levels, researchers can gain insight into the progression and prognosis of these conditions, paving the way for potential diagnostic and therapeutic advancements.Overall, the Human DMBT1 ELISA Kit offers a reliable and efficient solution for studying the role of DMBT1 in human health and disease, providing valuable data for further research and clinical applications.

Product Name:Human Deleted In Malignant Brain Tumors 1 (DMBT1) ELISA Kit
Product Code:HUDL00843
Size:96 Assays
Target:DMBT1
Synonyms:GP340, SAG, Muclin, Glycoprotein 340, Hensin, Salivary agglutinin, Surfactant pulmonary-associated D-binding protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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