The Human DEF β2 (Defensin Beta 2) ELISA Kit is specifically designed for the accurate quantification of defensin beta 2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a wide variety of research applications.Defensin beta 2 is a critical antimicrobial peptide that plays a key role in the innate immune system's defense against pathogens.
It has been implicated in various inflammatory and infectious diseases, making it a valuable biomarker for studying these conditions and evaluating potential therapeutic interventions.With its advanced technology and reliable performance, the Human DEF β2 (Defensin Beta 2) ELISA Kit is an essential tool for researchers looking to investigate the role of defensin beta 2 in health and disease.
Product Name:
Human DEF beta2/DEFB2 (Defensin Beta 2) ELISA Kit
SKU:
HUES02108
Target:
Human DEF beta2/DEFB2 (Defensin Beta 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DEFβ2/DEFB2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFβ2/DEFB2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFβ2/DEFB2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DEFβ2/DEFB2. You can calculate the concentration of Human DEFβ2/DEFB2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
97-112
88-100
Average (%)
91
105
94
1:4
Range (%)
91-103
88-102
83-94
Average (%)
97
93
89
1:8
Range (%)
88-103
85-99
84-95
Average (%)
94
92
88
1:16
Range (%)
93-109
85-98
86-100
Average (%)
100
92
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-100
94
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
88-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
182.36
364.62
1818.71
180.04
341.12
1735.26
Standard deviation
12.73
17.76
88.39
12.46
17.5
57.26
C V (%)
6.98
4.87
4.86
6.92
5.13
3.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DEFβ2/DEFB2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DEFβ2/DEFB2 in samples. No significant cross-reactivity or interference between Human DEFβ2/DEFB2 and analogues was observed.
