The Human DEFB2 (Defensin Beta 2) CLIA Kit is a highly reliable and accurate tool for measuring DEFB2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing researchers to obtain precise and consistent results for various research purposes.DEFB2, also known as Beta-Defensin 2, is a key component of the innate immune system, playing a crucial role in host defense against pathogens.
Its role in modulating immune responses and inflammation makes it a valuable biomarker for studying infectious diseases, inflammatory conditions, and other immune-related disorders.By using the Human DEFB2 CLIA Kit, researchers can gain valuable insights into the role of DEFB2 in various physiological and pathological processes, enabling advancements in understanding and potentially treating conditions related to immune function and inflammation.
Product Name:
Human DEF beta2/DEFB2 (Defensin Beta 2) CLIA Kit
SKU:
HUES00588
Target:
Human DEF beta2/DEFB2 (Defensin Beta 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DEFβ2/DEFB2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFβ2/DEFB2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFβ2/DEFB2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human DEFβ2/DEFB2. You can calculate the concentration of Human DEFβ2/DEFB2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
88-98
103-119
Average (%)
99
93
110
1:4
Range (%)
103-118
89-102
93-110
Average (%)
109
94
100
1:8
Range (%)
98-113
98-111
86-100
Average (%)
106
104
92
1:16
Range (%)
99-115
97-115
87-99
Average (%)
105
105
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-117
107
EDTA plasma (n=5)
96-113
103
Cell culture media (n=5)
96-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.88
120.01
365.52
47.55
127.94
355.59
Standard deviation
4.77
11.88
25.81
5.12
13.79
32.61
C V (%)
9.96
9.9
7.06
10.77
10.78
9.17
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human DEFβ2/DEFB2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DEFβ2/DEFB2 in samples. No significant cross-reactivity or interference between Human DEFβ2/DEFB2 and analogues was observed.
