Decorin is a secreted chondroitin/dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). SLRP family members are characterized by N-terminal and C-terminal cysteine-rich regions which flank the central region containing 10-12 tandem leucine-rich repeats (LRR). The human Decorin cDNA encodes a 359 amino acid (aa) precursor that includes a 16 aa signal sequence and a 14 aa propeptide. Alternate splicing of human Decorin generates five isoforms with variable length deletions. Decorin is an N-glycosylated protein that also carries a variablysized hybrid chondroitin/dermatan sulfate chain at Ser34. Decorin regulates assembly of the extracellular collagen matrix and the bioactivity of the matrix associated growth factors FGF2;GDF8/Myostatin; TGFβ;and WISP1. It also binds and activates EGF R; ErbB4; and IGFI-R. In vivo; Decorin promotes myoblast differentiation; supports angiogenesis; and inhibits tumor progression.
Product Name:
Human Decorin Recombinant Protein (RPES3154)
Product Code:
RPES3154
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
Decorin, Bone proteoglycan II, PG-S2, PG40, DCN, SLRR1B
Accession:
P07585
Sequence:
Gly17-Lys359
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Decorin is produced by our Mammalian expression system and the target gene encoding Gly17-Lys359 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
38.8 kDa
AP Mol Mass:
43-50 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS; pH7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
