Human DDT Recombinant Protein (RPPB1563)
- SKU:
- RPPB1563
- Product type:
- Recombinant Protein
- Size:
- 25ug
- Species:
- Human
- Target:
- DDT
- Synonyms:
- EC 41184
- DDCT
- D-dopachtome decarboxylase
- D-Dopachrome Tautomerase
- Source:
- Escherichia Coli
- Uniprot:
- P30046
Description
| Product Name: | Human DDT Recombinant Protein |
| Product Code: | RPPB1563 |
| Size: | 25µg |
| Species: | Human |
| Target: | DDT |
| Synonyms: | EC 4.1.1.84, DDCT, D-dopachtome decarboxylase, D-Dopachrome Tautomerase. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | DDT Human solution containing 20mM Tris-HCl pH-8, & 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPFLELDTNL PANRVPAGLE KRLCAAAASI LGKPADRVNV TVRPGLAMAL SGSTEPCAQL SISSIGVVGT AEDNRSHSAH FFEFLTKELA LGQDRILIRF FPLESWQIGK IGTVMTFL |
DDT is an enzyme that catayzes the tautomerization of D-dopachrome to give 5,6-dihydroxyindole (DHI). DDT is part of the family of lyases, specifically the carboxy-lyases, which cleave carbon-carbon bonds. DDT shares a homologous amino acid sequence (33% identical) with MIF and has similar tautomerase activity. DDT functions a proinflammatory cytokine.
DDT Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 138 amino acids (1-118 a.a.) and having a molecular mass of 14.8 kDa. The DDT is fused to a 20 amino acids His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DDT: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). Belongs to the MIF family. |
| UniProt Protein Details: | Protein type:EC 4.1.1.84; Lyase Chromosomal Location of Human Ortholog: 22q11.23 Cellular Component: cytoplasm Molecular Function:D-dopachrome decarboxylase activity; dopachrome isomerase activity Biological Process: melanin biosynthetic process |
| NCBI Summary: | D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30046 |
| NCBI GenInfo Identifier: | 2828192 |
| NCBI Gene ID: | 1652 |
| NCBI Accession: | P30046.3 |
| UniProt Secondary Accession: | P30046,O00774, O60787, Q13534, B7Z522, |
| UniProt Related Accession: | P30046 |
| Molecular Weight: | 11,323 Da |
| NCBI Full Name: | D-dopachrome decarboxylase |
| NCBI Synonym Full Names: | D-dopachrome tautomerase |
| NCBI Official Symbol: | DDT |
| NCBI Official Synonym Symbols: | DDCT |
| NCBI Protein Information: | D-dopachrome decarboxylase; phenylpyruvate tautomerase II |
| UniProt Protein Name: | D-dopachrome decarboxylase |
| UniProt Synonym Protein Names: | D-dopachrome tautomerase; Phenylpyruvate tautomerase II |
| Protein Family: | D-dopachrome decarboxylase |
| UniProt Gene Name: | DDT |
| UniProt Entry Name: | DOPD_HUMAN |
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