The Human DAO (Diamine Oxidase) ELISA Kit is specifically designed for the precise and accurate measurement of diamine oxidase levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results that are suitable for a variety of research purposes.Diamine oxidase is an important enzyme involved in the metabolism of histamine and other biogenic amines, with low levels of DAO being associated with conditions such as histamine intolerance and gastrointestinal disorders.
By accurately measuring DAO levels, researchers can better understand the role of this enzyme in various health conditions and potentially develop targeted treatments.Overall, the Human DAO (Diamine Oxidase) ELISA Kit provides researchers with a valuable tool for studying the role of diamine oxidase in human health and disease, paving the way for new insights and potential therapeutic advancements.
Product Name:
Human DAO (Diamine Oxidase) ELISA Kit
SKU:
HUES02305
Target:
Human DAO (Diamine Oxidase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human DAO. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DAO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DAO, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human DAO. You can calculate the concentration of Human DAO in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
89-101
95-109
Average (%)
99
94
101
1:4
Range (%)
87-103
85-100
88-103
Average (%)
94
91
95
1:8
Range (%)
88-105
86-100
88-104
Average (%)
96
92
95
1:16
Range (%)
89-101
82-96
82-96
Average (%)
94
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.74
28.26
90.38
10.95
27.59
89.37
Standard deviation
0.68
1.29
3.07
0.64
1.43
3.93
C V (%)
6.33
4.56
3.4
5.84
5.18
4.4
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human DAO concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human DAO in samples. No significant cross-reactivity or interference between Human DAO and analogues was observed.
