CP24,CYP24,P450-CC24,Vitamin D 24-Hydroxylase,1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial,Cytochrome P450 Family 24 Subfamily A Polypeptide 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Human Cytochrome P450 24A1 (CYP24A1) ELISA Kit is specifically designed for the quantitative detection of CYP24A1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Cytochrome P450 24A1 is a key enzyme involved in vitamin D metabolism, playing a crucial role in maintaining calcium homeostasis and bone health. Dysregulation of CYP24A1 has been linked to various diseases such as hypercalcemia, osteoporosis, and certain types of cancer.
This ELISA kit provides researchers with a valuable tool to study the role of CYP24A1 in these conditions and explore potential therapeutic interventions.Overall, the Human Cytochrome P450 24A1 (CYP24A1) ELISA Kit is an essential resource for investigating the biology and clinical implications of CYP24A1, offering accurate and reliable quantification of this important enzyme.
Product Name:
Human Cytochrome P450 24A1 (CYP24A1) ELISA Kit
Product Code:
HUDL00763
Size:
96 Assays
Target:
CYP24A1
Synonyms:
CP24, CYP24, P450-CC24, Vitamin D 24-Hydroxylase, 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial , Cytochrome P450 Family 24 Subfamily A Polypeptide 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
