Human Cytochrome C Oxidase Subunit II (COX2) ELISA Kit
The Human Cytochrome C Oxidase Subunit II (COX2) ELISA Kit is a powerful tool for accurately measuring levels of COX2 in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit provides consistent and reliable results, making it an invaluable resource for a variety of research applications.COX2 is a key enzyme involved in the electron transport chain and plays a critical role in energy production within mitochondria. Dysregulation of COX2 has been linked to various diseases, including metabolic disorders, neurodegenerative conditions, and cancer.
By quantifying COX2 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic strategies.Overall, the Human Cytochrome C Oxidase Subunit II (COX2) ELISA Kit offers a precise and efficient method for studying the role of COX2 in health and disease, ultimately advancing our understanding of mitochondrial function and its implications for human health.
Product Name:
Human Cytochrome C Oxidase Subunit II (COX2) ELISA Kit
Product Code:
HUDL00643
Size:
96 Assays
Target:
COX2
Synonyms:
MTCO2, MT-CO2, COII, COXII, Mitochondrially Encoded Cytochrome C Oxidase II, Cytochrome c oxidase polypeptide II
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
