Human Cytochrome b-245 Beta Polypeptide (CYBb) ELISA Kit

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SKU:
HUDL00753
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04839
Sensitivity:
0.055ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CGD,GP91PHOX,NOX2,Neutrophil cytochrome b91,Cytochrome b558 beta,Heme-binding membrane glycoprotein gp91phox,Superoxide-generating NADPH oxidase heavy chain
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Human Cytochrome b-245 Beta Polypeptide (CYBb) ELISA Kit

The Human Cytochrome b-245 beta Polypeptide (CYBB) ELISA Kit is a highly reliable tool for the precise measurement of CYBB levels in human samples including serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable asset for various research purposes.CYBB is a key component of the phagocyte NADPH oxidase system, essential for the production of reactive oxygen species involved in various biological processes such as immune defense and inflammation.

Dysregulation of CYBB has been implicated in disorders like chronic granulomatous disease and cardiovascular diseases, underscoring its importance as a biomarker for studying these conditions and developing potential therapeutic interventions.Get your Human Cytochrome b-245 beta Polypeptide (CYBB) ELISA Kit today and unlock new insights into the role of CYBB in health and disease.

Product Name:Human Cytochrome b-245 Beta Polypeptide (CYBb) ELISA Kit
Product Code:HUDL00753
Size:96 Assays
Target:CYBb
Synonyms:CGD, GP91PHOX, NOX2, Neutrophil cytochrome b91, Cytochrome b558 beta, Heme-binding membrane glycoprotein gp91phox, Superoxide-generating NADPH oxidase heavy chain
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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