Human Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit
The Human Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit is a cutting-edge diagnostic tool specifically designed for the precise measurement of CFTR levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, allowing for accurate and reproducible results that are crucial for various research applications.CFTR is a vital protein that plays a crucial role in regulating chloride and water movement in cells, especially in the lungs and digestive system.
Mutations in the CFTR gene are associated with cystic fibrosis, a genetic disorder that affects the respiratory and digestive systems. This ELISA kit provides researchers with a reliable method to study CFTR levels, aiding in the understanding of cystic fibrosis and the development of potential treatments for this debilitating condition.With its advanced technology and proven performance, the Human CFTR ELISA Kit is a valuable tool for researchers and healthcare professionals working towards improved diagnostics and therapies for cystic fibrosis.
Product Name:
Human Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
