Human Cysteinyl Leukotriene Receptor 1 (CYSLTR1) ELISA Kit
The Human Cysteinyl-Leukotriene Receptor 1 (CYSLTR1) ELISA Kit is a highly accurate and reliable tool for the detection of CYSLTR1 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and reproducible results, making it ideal for a variety of research applications.CYSLTR1 is a key receptor involved in the leukotriene pathway, playing a crucial role in inflammatory responses and allergic reactions. Dysregulation of CYSLTR1 has been linked to various diseases including asthma, allergic rhinitis, and inflammatory conditions, making it a valuable biomarker for studying these disorders and potential therapeutic interventions.
By utilizing the Human CYSLTR1 ELISA Kit, researchers can gain valuable insights into the role of CYSLTR1 in disease pathogenesis and treatment, paving the way for new discoveries and advancements in personalized medicine. Get accurate and reliable results with the Human CYSLTR1 ELISA Kit and accelerate your research today.
Product Name:
Human Cysteinyl Leukotriene Receptor 1 (CYSLTR1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
