Human Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) ELISA Kit
The Human CYR61 (Cysteine-rich protein 61) ELISA Kit is specifically designed for the precise detection of CYR61 levels in human serum, plasma, and cell culture supernatants. This advanced kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results for a variety of research purposes.CYR61 is a key protein known for its role in angiogenesis, serving as an inducer of blood vessel formation and affecting cell growth. Its significance in conditions such as cancer, cardiovascular diseases, and neurodegenerative disorders underscores its value as a vital biomarker for investigating these diseases and exploring potential treatment options.
Get your Human CYR61 ELISA Kit today and unlock valuable insights into the intricate processes of angiogenesis and cell proliferation. Trust in the precision and reliability of this kit to drive your research forward and uncover new discoveries in the field of molecular biology.
Product Name:
Human Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) ELISA Kit
Product Code:
HUDL00777
Size:
96 Assays
Target:
CYR61
Synonyms:
CCN1, GIG1, IGFBP10, CCN family member 1, Insulin-like growth factor-binding protein 10
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-99
94
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
