Human Cysteine And Glycine Rich Protein 1 (CSRP1) ELISA Kit
The Human CSRP1 (Cysteine and Glycine-rich Protein 1) ELISA Kit is specifically designed for the precise measurement of CSRP1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers consistent and accurate results, making it a valuable tool for various research purposes.CSRP1 is a protein known for its role in cell adhesion, cytoskeleton organization, and gene regulation. It plays a crucial part in various physiological processes and has been implicated in diseases such as cancer, cardiovascular disorders, and musculoskeletal conditions.
Therefore, studying CSRP1 levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of potential therapeutic interventions.Overall, the Human CSRP1 ELISA Kit offers a reliable and efficient way to quantify CSRP1 levels, making it a valuable resource for researchers investigating the implications of this protein in health and disease.
Product Name:
Human Cysteine And Glycine Rich Protein 1 (CSRP1) ELISA Kit
Product Code:
HUDL00702
Size:
96 Assays
Target:
CSRP1
Synonyms:
CRP1, CRP, CSRP, CYRP, HEL-141, Cysteine-rich protein 1, Epididymis luminal protein 141
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
