Human Cys-C (high-sensitivity Cystatin C) ELISA Kit
The Human Cys-C (Cystatin C) High Sensitivity ELISA Kit is specifically designed for the precise measurement of cystatin C levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and accuracy, ensuring consistent and dependable results, making it an excellent choice for various research applications.Cystatin C is a key protein involved in regulating kidney function and is widely used as a biomarker for kidney disease and renal dysfunction.
Elevated levels of cystatin C have also been linked to cardiovascular diseases, diabetes, and neurodegenerative disorders, highlighting its importance in understanding and managing these health conditions.By using the Human Cys-C High Sensitivity ELISA Kit, researchers can efficiently study the role of cystatin C in various diseases and develop potential diagnostic and therapeutic strategies for improved patient care.
Product Name:
Human Cys-C (high-sensitivity Cystatin C) ELISA Kit
SKU:
HUES02791
Target:
Human Cys-C (high-sensitivity Cystatin C)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human hs-Cys-C. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human hs-Cys-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human hs-Cys-C, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human hs-Cys-C. You can calculate the concentration of Human hs-Cys-C in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-105
87-102
95-110
Average (%)
100
93
102
1:4
Range (%)
87-98
83-94
83-95
Average (%)
92
88
89
1:8
Range (%)
92-106
81-93
87-101
Average (%)
99
86
94
1:16
Range (%)
90-105
82-97
85-100
Average (%)
98
89
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
94-111
102
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.49
4.28
13.99
1.54
4.08
15.02
Standard deviation
0.1
0.25
0.51
0.09
0.2
0.74
C V (%)
6.71
5.84
3.65
5.84
4.9
4.93
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human hs-Cys-C concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human hs-Cys-C in samples. No significant cross-reactivity or interference between Human hs-Cys-C and analogues was observed.
