The Human CYPB (Cyclophilin B) CLIA Kit is a highly reliable and accurate tool for the quantitative detection of Cyclophilin B levels in human biological samples such as serum, plasma, and cell culture supernatants. This CLIA kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Cyclophilin B, also known as CypB, is an important protein involved in various cellular processes, including protein folding, immune response modulation, and viral infection.
Dysregulation of Cyclophilin B has been linked to several diseases, making it a valuable biomarker for disease prognosis and therapeutic research.This CLIA kit provides researchers with a powerful tool to study the role of Cyclophilin B in health and disease, offering insights into potential therapeutic targets and diagnostic markers. Its ease of use and accurate results make it an essential asset for any laboratory focused on understanding Cyclophilin B and its implications in human health.
Product Name:
Human CYPB (Cyclophilin B) CLIA Kit
SKU:
HUES01065
Target:
Human CYPB (Cyclophilin B)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYPB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYPB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYPB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CYPB. You can calculate the concentration of Human CYPB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
86-98
99-111
Average (%)
93
93
105
1:4
Range (%)
94-110
88-101
88-102
Average (%)
101
94
94
1:8
Range (%)
92-106
89-106
94-108
Average (%)
98
96
100
1:16
Range (%)
88-98
92-106
93-106
Average (%)
93
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
99
EDTA plasma (n=5)
84-97
91
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
200.6
418.99
1835.95
186.21
405.29
1704.59
Standard deviation
22.67
41.27
144.31
16.57
48.43
114.04
C V (%)
11.3
9.85
7.86
8.9
11.95
6.69
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CYPB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CYPB in samples. No significant cross-reactivity or interference between Human CYPB and analogues was observed.
