CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.
Product Name:
Human CXADR/CAR Recombinant Protein (RPES0891)
Product Code:
RPES0891
Size:
50µg
Species:
Human
Expressed Host:
HEK293 Cells
Synonyms:
Coxsackievirus and Adenovirus Receptor, CAR, hCAR, CVB3-Binding Protein, Coxsackievirus B-Adenovirus Receptor, HCVADR, CXADR, CAR
Accession:
NP_001329.1
Sequence:
Met 1-Gly 237
Fusion tag:
C-His & Fc
Activity:
Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 5 x 10E4 cells/well are added to CXADR coated plates (4 µg/ml and 100 ?l/well), approximately 20%-40% will adhere specifically after 60 minutes at 37?.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the human CXADR (NP_001329.1) extracellular domain (Met 1-Gly 237) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Purity:
> 92 % as determined by reducing SDS-PAGE.
Mol Mass:
52 kDa
AP Mol Mass:
60-65 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
