Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit
The Human CX3CL1 Chemokine C-X3-C Motif Ligand-1 ELISA Kit is specifically designed for the accurate detection of CX3CL1 levels in human biological samples, including serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it suitable for a wide range of research applications.CX3CL1, also known as fractalkine, is a unique chemokine that exists in both soluble and membrane-bound forms. It plays a crucial role in immune responses, inflammation, and neuronal communication.
Dysregulation of CX3CL1 has been implicated in various diseases, including inflammatory disorders, neurodegenerative diseases, and cancer.By accurately measuring CX3CL1 levels, researchers can gain insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets. The Human CX3CL1 ELISA Kit provides a valuable tool for studying the role of CX3CL1 in health and disease, advancing our understanding of its biological functions and potential clinical applications.
Product Name:
Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) ELISA Kit
SKU:
HUES01331
Target:
Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CX3CL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CX3CL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CX3CL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CX3CL1. You can calculate the concentration of Human CX3CL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
99-115
89-100
Average (%)
97
105
94
1:4
Range (%)
87-99
84-94
90-102
Average (%)
93
89
95
1:8
Range (%)
88-102
84-96
82-97
Average (%)
95
89
88
1:16
Range (%)
90-102
85-98
85-98
Average (%)
96
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
94
EDTA plasma (n=5)
85-99
92
Cell culture media (n=5)
90-105
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.54
3.68
0.52
1.63
3.64
Standard deviation
0.03
0.09
0.14
0.03
0.07
0.18
C V (%)
5.88
5.84
3.8
5.77
4.29
4.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CX3CL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CX3CL1 in samples. No significant cross-reactivity or interference between Human CX3CL1 and analogues was observed.
