Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) CLIA Kit
The Human CX3CL1 (Chemokine C-X3-C motif ligand 1) CLIA Kit is a cutting-edge assay designed for the precise measurement of CX3CL1 levels in human biological samples. This kit offers unparalleled accuracy and reliability, allowing for consistent and reproducible results in a variety of research settings. CX3CL1, also known as fractalkine, is a unique chemokine involved in immune response and inflammation. It plays a crucial role in the recruitment and activation of immune cells, as well as in the regulation of cell adhesion and migration. Dysregulation of CX3CL1 has been implicated in various diseases, including autoimmune disorders, cardiovascular diseases, and neuroinflammatory conditions, highlighting its potential as a valuable biomarker for disease monitoring and therapeutic development.
With its advanced technology and superior performance, the Human CX3CL1 CLIA Kit is an essential tool for researchers studying the intricate mechanisms of immune response and inflammation, providing valuable insights into the pathophysiology of various diseases and guiding the development of innovative treatments.
Product Name:
Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1) CLIA Kit
SKU:
HUES00043
Target:
Human CX3CL1 (Chemokine C-X3-C-Motif Ligand 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CX3CL1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CX3CL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CX3CL1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human CX3CL1. You can calculate the concentration of Human CX3CL1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-121
90-102
101-114
Average (%)
110
96
108
1:4
Range (%)
100-115
86-97
88-102
Average (%)
106
91
96
1:8
Range (%)
100-115
83-98
99-112
Average (%)
106
90
105
1:16
Range (%)
85-97
99-114
89-106
Average (%)
90
106
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-114
108
EDTA plasma (n=5)
83-97
90
Cell culture media (n=5)
102-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
243.92
519.35
1729.51
238.98
551.97
1838.84
Standard deviation
22.64
58.69
109.48
20.38
46.2
125.59
C V (%)
9.28
11.3
6.33
8.53
8.37
6.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human CX3CL1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CX3CL1 in samples. No significant cross-reactivity or interference between Human CX3CL1 and analogues was observed.
