The Human CVL/EZR (Cytovillin/Ezrin) ELISA Kit is specifically designed for the accurate measurement of CVL/EZR levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.CVL/EZR, also known as Cytovillin and Ezrin, is a key protein involved in cell structure and function, playing a crucial role in cell adhesion, migration, and signaling pathways. Dysregulation of CVL/EZR has been linked to various diseases including cancer, inflammatory disorders, and infectious diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its reliable performance and ease of use, the Human CVL/EZR ELISA Kit is a valuable tool for researchers seeking to accurately quantify CVL/EZR levels in human samples to further their understanding of disease mechanisms and potential treatment strategies.
Product Name:
Human CVL/EZR (Cytovillin/Ezrin) ELISA Kit
SKU:
HUES02990
Target:
Human CVL/EZR (Cytovillin/Ezrin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CVL/EZR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CVL/EZR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CVL/EZR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CVL/EZR. You can calculate the concentration of Human CVL/EZR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
94-111
93-108
Average (%)
92
102
101
1:4
Range (%)
87-103
83-95
88-98
Average (%)
94
88
93
1:8
Range (%)
92-106
84-96
85-96
Average (%)
99
90
91
1:16
Range (%)
92-107
81-93
85-99
Average (%)
98
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-99
93
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.96
291.52
810.37
86.93
268.47
805.77
Standard deviation
6.59
16.44
26.74
4.82
14.44
36.42
C V (%)
6.94
5.64
3.3
5.54
5.38
4.52
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CVL/EZR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CVL/EZR in samples. No significant cross-reactivity or interference between Human CVL/EZR and analogues was observed.
