Human Cutaneous T-Cell Attracting Chemokine (CTACK) ELISA Kit
SKU:
HUDL00711
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Y4X3
Sensitivity:
10.2pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL27,ILC,SCYA27,ALP,Skinkine,ESkine,PESKY,CTAK,Chemokine(C-C-Motif)ligand 27,Small Inducible Cytokine Subfamily A Member 27,IL-11 R-alpha-locus chemokine
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Human Cutaneous T-Cell Attracting Chemokine (CTACK) ELISA Kit
The Human Cutaneous T-Cell Attracting Chemokine (CTACK) ELISA Kit is specifically designed for the precise measurement of CTACK levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and reproducible results, making it a valuable tool for a variety of research applications.CTACK is a key chemokine that plays a vital role in immune cell recruitment to the skin, contributing to the regulation of inflammatory responses and immune surveillance.
Dysregulation of CTACK has been implicated in several skin disorders, autoimmune diseases, and inflammatory conditions, highlighting its significance as a biomarker for studying these conditions and identifying novel therapeutic strategies.Overall, the Human CTACK ELISA Kit allows researchers to delve deeper into the molecular mechanisms underlying immune responses in the skin, paving the way for advancements in dermatology, immunology, and drug development.
Product Name:
Human Cutaneous T-Cell Attracting Chemokine (CTACK) ELISA Kit
Product Code:
HUDL00711
Size:
96 Assays
Target:
CTACK
Synonyms:
CCL27, ILC, SCYA27, ALP, Skinkine, ESkine, PESKY, CTAK, Chemokine(C-C-Motif)ligand 27, Small Inducible Cytokine Subfamily A Member 27, IL-11 R-alpha-locus chemokine
Detection Method:
Sandwich
Range:
31.2-2,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
