Human CUB Domain Containing Protein 1 (CDCP1) ELISA Kit
SKU:
HUDL00503
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9H5V8
Sensitivity:
0.127ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD318,CDCP1,SIMA135,TRASK,Membrane glycoprotein gp140,Transmembrane and associated with src kinases,Subtractive immunization M plus HEp3-associated 135 kDa
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human CUB Domain Containing Protein 1 (CDCP1) ELISA Kit
The Human CDCP1 (Cub Domain Containing Protein 1) ELISA Kit is specifically designed for the precise and sensitive detection of CDCP1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit is known for its high accuracy and specificity, ensuring consistent and dependable results for a variety of research applications.CDCP1 is a protein that plays a key role in cancer progression and metastasis, making it a valuable biomarker for studying cancer biology and developing targeted therapies.
By measuring CDCP1 levels, researchers can gain valuable insights into the mechanisms of cancer development and progression, facilitating the development of novel treatment strategies for cancer patients.Overall, the Human CDCP1 ELISA Kit offers researchers a powerful tool for studying the role of CDCP1 in cancer and other diseases, providing valuable data for advancing our understanding of these conditions and ultimately improving patient outcomes.
Product Name:
Human CUB Domain Containing Protein 1 (CDCP1) ELISA Kit
Product Code:
HUDL00503
Size:
96 Assays
Target:
CDCP1
Synonyms:
CD318, CDCP1, SIMA135, TRASK, Membrane glycoprotein gp140, Transmembrane and associated with src kinases, Subtractive immunization M plus HEp3-associated 135 kDa
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
