The Human CTSD (Cathepsin D) ELISA Kit is specifically designed for the precise measurement of cathepsin D levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an excellent tool for various research applications.Cathepsin D is a key enzyme involved in the breakdown of proteins within lysosomes, playing a vital role in various cellular processes such as autophagy, apoptosis, and cell proliferation.
Dysregulation of cathepsin D has been implicated in numerous diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.Overall, the Human CTSD (Cathepsin D) ELISA Kit offers researchers a reliable and efficient method for quantifying cathepsin D levels, facilitating groundbreaking discoveries and advancements in the field of biomedical research.
Product Name:
Human CTSD (Cathepsin D) ELISA Kit
SKU:
HUES01825
Target:
Human CTSD (Cathepsin D)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
28.13 pg/mL
Detection range:
46.88-3000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTSD. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTSD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTSD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTSD. You can calculate the concentration of Human CTSD in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
86-99
92-105
Average (%)
92
92
100
1:4
Range (%)
89-101
81-95
89-102
Average (%)
94
87
95
1:8
Range (%)
91-106
86-97
84-97
Average (%)
98
91
90
1:16
Range (%)
92-106
86-99
86-102
Average (%)
98
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-108
98
EDTA plasma (n=5)
93-110
101
Cell culture media (n=5)
96-111
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
159.19
309.14
1474.61
150.32
320.64
1539.94
Standard deviation
8.68
16.76
78.6
9.88
17.57
63.45
C V (%)
5.45
5.42
5.33
6.57
5.48
4.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CTSD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTSD in samples. No significant cross-reactivity or interference between Human CTSD and analogues was observed.
