The Human CTNNB1 (Beta-Catenin) ELISA Kit is specifically designed for precise measurement of beta-catenin levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research purposes.Beta-catenin is a key component in the Wnt signaling pathway and serves as a crucial regulator of cell proliferation, differentiation, and development. Dysregulation of beta-catenin has been implicated in various diseases including cancer, inflammatory disorders, and developmental abnormalities, making it a valuable biomarker for studying these conditions and exploring potential therapeutic targets.
With its advanced technology and reliable performance, the Human CTNNB1 ELISA Kit from Assay Genie is an essential tool for researchers seeking to unravel the role of beta-catenin in health and disease. Get your kit today and unlock new insights into this vital signaling protein.
Product Name:
Human CTNN beta1 (Catenin, Beta 1) ELISA Kit
SKU:
HUES01824
Target:
Human CTNN beta1 (Catenin, Beta 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTNNβ1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTNNβ1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTNNβ1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTNNβ1. You can calculate the concentration of Human CTNNβ1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
93-104
98-110
Average (%)
93
98
104
1:4
Range (%)
86-102
83-96
84-94
Average (%)
93
90
89
1:8
Range (%)
90-103
81-94
86-99
Average (%)
98
87
92
1:16
Range (%)
93-105
83-95
87-100
Average (%)
98
90
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
94
EDTA plasma (n=5)
86-100
93
Cell culture media (n=5)
93-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
1.11
3.77
0.57
1.04
3.44
Standard deviation
0.04
0.05
0.19
0.03
0.05
0.13
C V (%)
7.55
4.5
5.04
5.26
4.81
3.78
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CTNNβ1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CTNNβ1 in samples. No significant cross-reactivity or interference between Human CTNNβ1 and analogues was observed.
