The Human CTn-I (cTnI, TNNI3) Cardiac Troponin I ELISA Kit is specifically designed for the precise measurement of cardiac troponin I levels in human samples such as serum, plasma, and cell culture supernatants. With its outstanding sensitivity and specificity, this kit ensures accurate and consistent results, making it ideal for various research applications in the field of cardiology.Cardiac troponin I is a key biomarker for assessing cardiac health and diagnosing conditions such as myocardial infarction (heart attack) and other cardiovascular diseases.
Monitoring CTn-I levels can help in early detection, risk stratification, and treatment evaluation in patients with heart-related issues. This ELISA kit provides researchers with a reliable tool for studying cardiovascular diseases and exploring potential treatment strategies.
Product Name:
Human cTn-I/TNNI3 (cardiac Troponin I) ELISA Kit
SKU:
HUES01810
Target:
Human cTn-I/TNNI3 (cardiac Troponin I)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNNI3/cTn-I. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNNI3/cTn-I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNNI3/cTn-I, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNNI3/cTn-I. You can calculate the concentration of Human TNNI3/cTn-I in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
90-103
91-102
Average (%)
103
96
96
1:4
Range (%)
96-110
86-100
90-106
Average (%)
104
93
98
1:8
Range (%)
97-113
80-94
91-105
Average (%)
105
86
99
1:16
Range (%)
100-112
84-97
95-111
Average (%)
106
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-101
96
EDTA plasma (n=5)
96-111
102
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.2
3.0
8.7
1.3
2.7
8.5
Standard deviation
0.1
0.2
0.4
0.1
0.1
0.4
C V (%)
8.33
6.67
4.6
7.69
3.7
4.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human TNNI3/cTn-I concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human TNNI3/cTn-I in samples. No significant cross-reactivity or interference between Human TNNI3/cTn-I and analogues was observed.
