Human CTF1 Recombinant Protein (RPPB0139)
- SKU:
- RPPB0139
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Human
- Target:
- CTF1
- Synonyms:
- CTF1
- CT1
- CT-1
- Cardiophin 1
- Source:
- Escherichia Coli
- Uniprot:
- Q16619
Description
| Product Name: | Human CTF1 Recombinant Protein |
| Product Code: | RPPB0139 |
| Size: | 10µg |
| Species: | Human |
| Target: | CTF1 |
| Synonyms: | CTF1, CT1, CT-1, Cardiophin 1, Cardiotrophin-1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | CTF-1 protein was lyophilized from a 0.2µm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA. |
| Solubility: | It is recommended to reconstitute the lyophilized CTF1 in sterile 4mM HCl not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized CTF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CTF-1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A |
| Biological Activity: | The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0×106 IU/mg. |
Cardiotrophin 1 (CT-1) is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT 1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT 1 activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes.CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins (hsps) in cardiac cells.Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide (BNP) and angiotensinogen mRNA.Cardiophin 1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.
Cardiotrophin-1 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 201 amino acids and having a molecular mass of 21.2kDa.The CTF1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CTF1: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cytokine Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: extracellular space; extracellular region Molecular Function:leukemia inhibitory factor receptor binding; cytokine activity Biological Process: cell proliferation; nervous system development; muscle development; cell-cell signaling; leukemia inhibitory factor signaling pathway; positive regulation of cell proliferation; neuron development |
| NCBI Summary: | The protein encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008] |
| UniProt Code: | Q16619 |
| NCBI GenInfo Identifier: | 2498265 |
| NCBI Gene ID: | 1489 |
| NCBI Accession: | Q16619.1 |
| UniProt Secondary Accession: | Q16619,Q5U5Y7, A8MVX4, |
| UniProt Related Accession: | Q16619 |
| Molecular Weight: | 21,098 Da |
| NCBI Full Name: | Cardiotrophin-1 |
| NCBI Synonym Full Names: | cardiotrophin 1 |
| NCBI Official Symbol: | CTF1 |
| NCBI Official Synonym Symbols: | CT1; CT-1 |
| NCBI Protein Information: | cardiotrophin-1; cardiophin 1 |
| UniProt Protein Name: | Cardiotrophin-1 |
| Protein Family: | Cardiotrophin |
| UniProt Gene Name: | CTF1 |
| UniProt Entry Name: | CTF1_HUMAN |
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