Human CRYAB Recombinant Protein (RPPB5194)

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SKU:
RPPB5194
Product type:
Recombinant Protein
Size:
100ug
Species:
Human
Target:
CRYAB
Synonyms:
CRYA2
Synonyms:
CTPP2
Synonyms:
HSPB5
Synonyms:
Crystallin Alpha B
Source:
Escherichia Coli
Uniprot:
P02511
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Description

Product Name:Human CRYAB Recombinant Protein
Product Code:RPPB5194
Size:100µg
Species:Human
Target:CRYAB
Synonyms:CRYA2, CTPP2, HSPB5, Crystallin Alpha B, CRYAB.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The CRYAB protein (1mg/ml) solution contains 20mM Tris-HCl buffer pH-7.5, 50mM NaCl and 1mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSWFDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHRKYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK

Alpha crystallins are composed of two gene products ; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of a-crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-B is expressed widely in many tissues and organs and occurs in many neurological diseases.

Recombinant CRYAB produced in E.Coli is a single,non-glycosylated polypeptide chain containing 175 amino acids and having a molecular mass of 20.1kDa. CRYAB is purified by proprietary chromatographic techniques.

UniProt Protein Function:CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
UniProt Protein Details:

Protein type:Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 11q22.3-q23.1

Cellular Component: actin filament bundle; cell surface; cytoplasm; cytosol; Golgi apparatus; microtubule cytoskeleton; mitochondrion; myelin sheath; nucleoplasm; nucleus; plasma membrane; Z disc

Molecular Function:identical protein binding; metal ion binding; microtubule binding; protein binding; protein homodimerization activity; structural constituent of eye lens; unfolded protein binding

Biological Process: aging; lens development in camera-type eye; microtubule polymerization or depolymerization; muscle contraction; muscle development; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of cell growth; negative regulation of intracellular transport; protein folding; protein homooligomerization; response to estradiol stimulus; response to hydrogen peroxide; response to hypoxia; stress-activated MAPK cascade; tubulin folding

Disease: Cardiomyopathy, Dilated, 1ii; Cataract 16, Multiple Types; Myopathy, Myofibrillar, 2; Myopathy, Myofibrillar, Fatal Infantile Hypertonic, Alpha-b Crystallin-related

NCBI Summary:Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
UniProt Code:P02511
NCBI GenInfo Identifier:117385
NCBI Gene ID:1410
NCBI Accession:P02511.2
UniProt Secondary Accession:P02511,O43416, Q9UC37, Q9UC38, Q9UC39, Q9UC40, Q9UC41 B0YIX0,
UniProt Related Accession:P02511
Molecular Weight:20,159 Da
NCBI Full Name:Alpha-crystallin B chain
NCBI Synonym Full Names:crystallin alpha B
NCBI Official Symbol:CRYAB  
NCBI Official Synonym Symbols:MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101  
NCBI Protein Information:alpha-crystallin B chain
UniProt Protein Name:Alpha-crystallin B chain
UniProt Synonym Protein Names:Alpha(B)-crystallin; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Protein Family:Alpha-crystallin
UniProt Gene Name:CRYAB  
UniProt Entry Name:CRYAB_HUMAN
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