The Human CRP (C-reactive protein) ELISA Kit is specifically designed for the precise measurement of CRP levels in human serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and accuracy, guaranteeing consistent and trustworthy results for a variety of research purposes.CRP is an important biomarker of inflammation and infection in the body, with elevated levels indicating potential health issues such as cardiovascular disease, autoimmune disorders, and chronic inflammatory conditions.
Monitoring CRP levels can provide valuable insights into the body's immune response and overall health status, making this ELISA kit an indispensable tool for researchers and healthcare professionals alike.
Product Name:
Human CRP (C-Reactive Protein) ELISA Kit
SKU:
HUES01330
Target:
Human CRP (C-Reactive Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.23 ng/mL
Detection range:
0.39-25 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
92-106
98-109
Average (%)
103
98
103
1:4
Range (%)
97-112
85-96
90-105
Average (%)
105
91
97
1:8
Range (%)
98-111
86-100
93-111
Average (%)
105
93
101
1:16
Range (%)
99-113
87-101
98-114
Average (%)
105
92
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
95
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
88-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.5
4.9
16.8
2.5
4.7
18.2
Standard deviation
0.1
0.2
0.6
0.2
0.3
0.7
C V (%)
4
4.08
3.57
8.0
6.38
3.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed.
