Human Cross Linked C-Telopeptide Of Type III Collagen (CTXIII) ELISA Kit
The Human Cross-Linked C-Telopeptide of Type III Collagen (CTXIII) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of CTXIII levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a variety of research applications.CTXIII is a marker of collagen degradation and is crucial for studying conditions related to bone metabolism, such as osteoporosis and osteoarthritis. By measuring CTXIII levels, researchers can gain valuable insights into the progression and treatment of these disorders, as well as monitor the effectiveness of therapeutic interventions.
With its user-friendly protocols and high-performance components, the Human Cross-Linked C-Telopeptide of Type III Collagen (CTXIII) ELISA Kit is an essential tool for researchers working in the fields of bone biology, musculoskeletal disorders, and connective tissue diseases. Start uncovering the secrets of collagen degradation with this advanced ELISA kit today.
Product Name:
Human Cross Linked C-Telopeptide Of Type III Collagen (CTXIII) ELISA Kit
Product Code:
HUDL00735
Size:
96 Assays
Target:
CTXIII
Synonyms:
CTX-III, CTX3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
