Creatine kinase B-type (CKB) belongs to the ATP:guanido phosphotransferase family. It has dimer of identical or non-identical chains with MM being the major form in skeletal muscle and myocardium. MB exists in myocardium, and BB exists in many tissues, especially brain. CKB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure.
Product Name:
Human Creatine Kinase BB/CKB Recombinant Protein (RPES3312)
Product Code:
RPES3312
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
B-CK, CKB, Creatine Kinase BB
Accession:
P12277
Sequence:
Met1-Lys381
Fusion tag:
N-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Creatine Kinase BB is produced by our E.coli expression system and the target gene encoding Met1-Lys381 is expressed with a 6His tag at the N-terminus.
Purity:
> 90% as determined by reducing SDS-PAGE.
Mol Mass:
44.8 kDa
AP Mol Mass:
50 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM TrisHCl,150mM NaCl,10% Glycerol,pH 7.5.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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