Death domain-containing protein CRADD; also known as Caspase and RIP adapter with death domain; RIP-associated protein with a death domain; CRADD and RAIDD; is a protein which is constitutively expressed in most tissues; with particularly high expression in adult heart; testis; liver; skeletal muscle; fetal liver and kidney. CRADD / RAIDD contains one CARD domain and one death domain. CRADD / RAIDD contains a death domain involved in the binding of RIP protein. The CARD domain mediates the interaction with caspase-2. FADD / MORT1 is a death domain (DD)-containing adaptor / signaling molecule that interacts with the intracellular DD of FAS / APO-I ( CD95 ) and tumor necrosis factor receptor 1 and the prodomain of caspase-8 ( Mch5 / MACH / FLICE). CRADD / RAIDD has a dual-domain structure similar to that of FADD. CRADD / RAIDD has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. CRADD / RAIDD could play a role in regulating apoptosis in mammalian cells. CRADD / RAIDD is a apoptotic adaptor molecule specific for caspase-2 and FASL / TNF receptor-interacting protein RIP. In the presence of RIP and TRADD; CRADD / RAIDD recruits caspase-2 to the TNFR-1 signalling complex.
Product Name:
Human CRADD/RAIDD Recombinant Protein (RPES1734)
Product Code:
RPES1734
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Death Domain-Containing Protein CRADD, Caspase and RIP Adapter with Death Domain, RIP-Associated Protein with A Death Domain, CRADD, RAIDD
Accession:
P78560
Sequence:
Met 1-Glu 199
Fusion tag:
C-His
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human CRADD (P78560) (Met 1-Glu 199) was fused with a polyhistidine tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
24.1 kDa
AP Mol Mass:
26 kDa
Formulation:
Lyophilized from sterile PBS; 20% glycerol; pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
