Cellular retinoic acid-binding protein 2, also known as Cellular retinoic acid-binding protein II, CRABP-II and CRABP2, is a protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The predicted amino acid sequences of human CRABP1 and CRABP2 demonstrated a 99.3% and 93.5% identity to mouse CRABP1 and CRABP2, respectively. CRABP2 forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. Expression of CRABP2, but not CRABP1 mRNA, was markedly increased (greater than 15-fold) by retinoic acid treatment of fibroblasts cultured from human skin, whereas no significant induction of CRABP2 mRNA was observed in human lung fibroblasts. CRABP2 transports retinoic acid to the nucleus. It regulates the access of retinoic acid to the nuclear retinoic acid receptors. CRABP2 is necessary for elastin induction by All-trans retinoic acid (ATRA) in MRC-5 cells. It is expressed at low levels in emphysema fibroblasts. This alteration in the retinoic acid signalling pathway in lung fibroblasts may contribute to the defect of alveolar repair in human pulmonary emphysema.
Product Name:
Human CRABP2 Recombinant Protein (RPES2922)
Product Code:
RPES2922
Size:
50µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
CRABP-II,RBP6
Accession:
NP_001869.1
Sequence:
Pro2-Glu 138
Fusion tag:
N-His
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human CRABP2 (NP_001869.1) (Pro2-Glu 138) was expressed, with a polyhistide tag at the N-terminus.
Purity:
> 96 % as determined by reducing SDS-PAGE.
Mol Mass:
16.5 kDa
AP Mol Mass:
16.5 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
