Human COX2 (Cytochrome C Oxidase Subunit II) ELISA Kit
The Human COX-2 (Cytochrome C Oxidase Subunit II) ELISA Kit is a specialized assay kit designed for the precise and accurate measurement of COX-2 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and consistent results, making it a valuable tool for a variety of research applications.COX-2, also known as cytochrome c oxidase subunit II, is an important enzyme involved in the inflammatory response and is associated with various diseases including cancer, arthritis, and inflammatory disorders.
By detecting and quantifying COX-2 levels, researchers can gain insights into the role of this enzyme in disease progression and potentially identify new therapeutic targets. Trust the Human COX-2 ELISA Kit for accurate and precise measurement of COX-2 levels in human samples, advancing your research in the field of inflammation and disease. For more information, visit www.assaygenie.com/human-cox2-cytochrome-c-oxidase-subunit-ii-elisa-kit-hues03548/
Product Name:
Human COX2 (Cytochrome C Oxidase Subunit II) ELISA Kit
SKU:
HUES03548
Target:
Human COX2 (Cytochrome C Oxidase Subunit II)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COX2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COX2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COX2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COX2. You can calculate the concentration of Human COX2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
91-101
92-104
Average (%)
101
96
99
1:4
Range (%)
88-103
87-99
86-99
Average (%)
94
93
92
1:8
Range (%)
88-98
83-92
88-103
Average (%)
93
87
94
1:16
Range (%)
94-107
83-97
82-93
Average (%)
100
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-102
97
EDTA plasma (n=5)
96-106
101
Cell culture media (n=5)
86-99
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
2.44
7.66
0.99
2.28
7.48
Standard deviation
0.05
0.12
0.26
0.05
0.11
0.23
C V (%)
5.1
4.92
3.39
5.05
4.82
3.07
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COX2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COX2 in samples. No significant cross-reactivity or interference between Human COX2 and analogues was observed.
