CLABP,p57,CLIPINA,HCORO1,TACO,Coronin-1,Coronin,Actin-Binding Protein,1A,Coronin-like protein A,Coronin-like protein p57,Tryptophan aspartate-containing coat protein
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
The Human Coronin-1A (Coro1A) ELISA Kit is specifically designed for the accurate detection of Coronin-1A levels in human serum, plasma, and cell culture supernatants. Coronin-1A is a key protein involved in cell migration and actin dynamics, making it an important factor in various cellular processes. This kit offers high sensitivity and specificity, ensuring dependable and consistent results for a wide range of research applications.
Coronin-1A is implicated in immune response, cell adhesion, and cytoskeleton organization, making it a valuable biomarker for studying these pathways and potential therapeutic targets. With its reliable performance and user-friendly protocol, the Human Coronin-1A (Coro1A) ELISA Kit is a valuable tool for researchers investigating the role of Coronin-1A in health and disease.
Product Name:
Human Coronin 1A (CORO1A) ELISA Kit
Product Code:
HUDL00639
Size:
96 Assays
Target:
CORO1A
Synonyms:
CLABP, p57, CLIPINA, HCORO1, TACO, Coronin-1, Coronin,Actin-Binding Protein,1A, Coronin-like protein A, Coronin-like protein p57, Tryptophan aspartate-containing coat protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
