The Human Complement Component 5 (C5) ELISA Kit is specifically designed for the precise measurement of complement component 5 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for a wide range of research applications.Complement component 5 is a vital protein involved in the complement system, playing a key role in the inflammatory response and immune defense mechanisms. Dysregulation of C5 has been implicated in various diseases such as autoimmune disorders, inflammatory conditions, and infectious diseases, making it an important biomarker for studying these conditions and potential therapeutic interventions.
By utilizing the Human Complement Component 5 (C5) ELISA Kit, researchers can gain valuable insights into the role of C5 in disease pathogenesis and progression, ultimately leading to advancements in diagnosis, prognosis, and treatment strategies. Order now to enhance your research with reliable and accurate measurements of complement component 5 levels.
Product Name:
Human Complement Component 5 (C5) ELISA Kit
Product Code:
HUDL00386
Size:
96 Assays
Target:
C5
Synonyms:
CPAMD4, C5a anaphylatoxin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
