Human COMP (Cartilage Oligomeric Matrix Protein) CLIA Kit
The Human COMP (Cartilage Oligomeric Matrix Protein) CLIA Kit is a cutting-edge diagnostic tool designed for the accurate measurement of COMP levels in human serum, plasma, and cell culture supernatants. This kit offers unrivaled sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.COMP is a key protein found in cartilage and synovial tissues, playing a critical role in maintaining joint health and function. Abnormal levels of COMP have been linked to various joint disorders and arthritis, making it a valuable biomarker for studying these conditions and identifying potential therapeutic targets.
Whether investigating cartilage degradation, monitoring disease progression, or developing new treatment strategies, the Human COMP CLIA Kit offers researchers a powerful tool for advancing their understanding of joint health and disease. Trust in the reliability and accuracy of this kit to drive your research forward.
Product Name:
Human COMP (Cartilage Oligomeric Matrix Protein) CLIA Kit
SKU:
HUES00433
Target:
Human COMP (Cartilage Oligomeric Matrix Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COMP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COMP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COMP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COMP. You can calculate the concentration of Human COMP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
88-99
99-117
Average (%)
106
94
107
1:4
Range (%)
91-104
99-114
88-102
Average (%)
98
107
94
1:8
Range (%)
97-111
101-114
101-115
Average (%)
104
108
107
1:16
Range (%)
92-106
102-117
90-105
Average (%)
99
108
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
102-117
108
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
95-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.03
4.15
0.48
1.11
3.9
Standard deviation
0.04
0.11
0.31
0.04
0.1
0.44
C V (%)
8.16
10.68
7.47
8.33
9.01
11.28
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COMP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COMP in samples. No significant cross-reactivity or interference between Human COMP and analogues was observed.
