Human Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit

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SKU:
HUDL01161
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P09919
Sensitivity:
5.4pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CSF3,G-CSF,Granulocyte Colony Stimulating Factor,Pluripoietin,Filgrastim,Lenograstim
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
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Description

system_update_altDatasheet

Human Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit

The Human Colony Stimulating Factor 3 (Granulocyte G-CSF) ELISA Kit is specifically developed for the precise measurement of Granulocyte Colony-Stimulating Factor 3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Granulocyte Colony-Stimulating Factor 3, also known as G-CSF, is a vital protein that plays a key role in stimulating the production and function of white blood cells, particularly granulocytes.

It is essential in regulating the immune response and can be critical in conditions such as infections, inflammatory diseases, and immune disorders. This ELISA kit provides a valuable tool for investigating the role of G-CSF in these conditions and exploring potential therapeutic interventions.

Product Name:Human Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit
Product Code:HUDL01161
Size:96 Assays
Target:GCSF
Synonyms:CSF3, G-CSF, Granulocyte Colony Stimulating Factor, Pluripoietin, Filgrastim, Lenograstim
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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