The Human Collagen Type VI alpha 3 (COL6A3) ELISA Kit is designed for the precise measurement of COL6A3 levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring accurate and consistent results for various research purposes.COL6A3 is a key component of the extracellular matrix, playing a critical role in maintaining tissue structure and function. Dysregulation of COL6A3 has been implicated in various diseases, including musculoskeletal disorders and connective tissue diseases.
Therefore, measuring COL6A3 levels can provide valuable insights into disease pathogenesis and therapeutic interventions.With its advanced technology and reliable performance, the Human Collagen Type VI alpha 3 (COL6A3) ELISA Kit is a valuable tool for researchers studying extracellular matrix disorders, tissue remodeling, and potential therapeutic targets.
Product Name:
Human Collagen Type VI Alpha 3 (COL6a3) ELISA Kit
Product Code:
HUDL00629
Size:
96 Assays
Target:
COL6a3
Synonyms:
COL6-A3, Collagen Alpha-3(VI)chain
Detection Method:
Sandwich
Range:
31.25-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
