The Human Collagen Type VI Alpha 1 (COL6A1) ELISA Kit is a powerful tool for the precise measurement of COL6A1 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it well-suited for a variety of research purposes.Collagen Type VI Alpha 1 is a key component of the extracellular matrix, playing a crucial role in maintaining tissue structure and integrity. Dysregulation of COL6A1 has been linked to various diseases, including muscular dystrophies, skin disorders, and connective tissue diseases.
By accurately measuring COL6A1 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human Collagen Type VI Alpha 1 (COL6A1) ELISA Kit offers a reliable and efficient means of studying the role of COL6A1 in health and disease, making it an invaluable tool for researchers in the biomedical and life sciences fields.
Product Name:
Human Collagen Type VI Alpha 1 (COL6a1) ELISA Kit
Product Code:
HUDL00628
Size:
96 Assays
Target:
COL6a1
Synonyms:
COL6-A1, OPLL, Collagen Alpha-1(VI)chain
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
