The Human COL7 (Collagen Type VII) ELISA Kit is a powerful tool for accurately measuring COL7 levels in human serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit provides dependable and consistent results, making it a valuable asset for various research endeavors.COL7 is a key component of anchoring fibrils in the skin, essential for maintaining the structural integrity of the skin's basement membrane.
Mutations in the COL7 gene can lead to genetic disorders such as dystrophic epidermolysis bullosa, highlighting the importance of monitoring COL7 levels in research related to skin diseases and wound healing.By utilizing the Human COL7 ELISA Kit, researchers can gain valuable insights into the role of COL7 in skin homeostasis and disease pathogenesis, ultimately paving the way for the development of novel therapeutic strategies.
Product Name:
Human COL7 (Collagen Type VII) ELISA Kit
SKU:
HUES01919
Target:
Human COL7 (Collagen Type VII)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL7. You can calculate the concentration of Human COL7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-112
93-106
91-103
Average (%)
102
100
98
1:4
Range (%)
99-117
84-99
96-110
Average (%)
107
90
102
1:8
Range (%)
99-116
87-102
99-114
Average (%)
106
94
105
1:16
Range (%)
99-109
87-102
93-105
Average (%)
104
93
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
97-110
102
Cell culture media (n=5)
87-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.2
4.3
0.5
1.3
4.5
Standard deviation
0.04
0.06
0.15
0.03
0.06
0.25
C V (%)
8
5.0
3.49
6.0
4.62
5.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL7 in samples. No significant cross-reactivity or interference between Human COL7 and analogues was observed.
