Human COL5 alpha2 (Collagen Type V Alpha 2) CLIA Kit
The Human Col5 Alpha2 (Collagen Type V Alpha 2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Col5 Alpha2 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.Collagen Type V Alpha 2 is a vital component of the extracellular matrix and plays a crucial role in maintaining tissue structure and integrity. It is particularly important in the formation of collagen fibrils and elastic fibers, contributing to the stability and flexibility of connective tissues.
Dysregulation of Col5 Alpha2 expression has been linked to various diseases, including Ehlers-Danlos syndrome and osteoarthritis, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Human Col5 Alpha2 CLIA Kit offers researchers a powerful tool for studying the role of Collagen Type V Alpha 2 in health and disease, providing insights into its impact on tissue development and maintenance.
Product Name:
Human COL5 alpha2 (Collagen Type V Alpha 2) CLIA Kit
SKU:
HUES00559
Target:
Human COL5 alpha2 (Collagen Type V Alpha 2)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human COL5α2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL5α2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL5α2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human COL5α2. You can calculate the concentration of Human COL5α2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
94-106
95-111
Average (%)
99
100
102
1:4
Range (%)
90-105
88-103
91-102
Average (%)
98
94
97
1:8
Range (%)
95-111
90-105
91-104
Average (%)
101
96
97
1:16
Range (%)
89-102
91-105
99-115
Average (%)
94
97
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
90
EDTA plasma (n=5)
94-111
102
Cell culture media (n=5)
93-109
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.55
15.16
45.91
5.3
15.65
47.4
Standard deviation
0.67
1.42
3.27
0.61
1.61
4.35
C V (%)
12.07
9.37
7.12
11.51
10.29
9.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human COL5α2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL5α2 in samples. No significant cross-reactivity or interference between Human COL5α2 and analogues was observed.
