The Human Col4 (Collagen Type IV) ELISA Kit is specifically designed for the precise measurement of collagen type IV levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.Collagen type IV is a key component of the basement membrane, crucial for maintaining tissue structure and integrity. Abnormal levels of collagen type IV have been linked to various diseases such as kidney diseases, diabetes, and autoimmune disorders, highlighting its importance as a potential biomarker for disease progression and therapeutic development.
This ELISA kit is a valuable tool for researchers studying collagen type IV biology, pathophysiology, and potential therapeutic interventions. With its reliable performance and ease of use, this kit is an essential addition to any lab looking to further explore the role of collagen type IV in human health and disease.
Product Name:
Human COL4 (Collagen Type IV) ELISA Kit
SKU:
HUES01445
Target:
Human COL4 (Collagen Type IV)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL4. You can calculate the concentration of Human COL4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-104
87-99
95-109
Average (%)
95
93
101
1:4
Range (%)
87-99
87-99
87-100
Average (%)
94
93
94
1:8
Range (%)
92-105
84-96
81-95
Average (%)
98
89
88
1:16
Range (%)
91-105
81-94
84-97
Average (%)
96
88
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
93
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
86-102
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.47
4.27
17.11
2.66
4.21
18.63
Standard deviation
0.16
0.23
0.53
0.17
0.19
0.87
C V (%)
6.48
5.39
3.1
6.39
4.51
4.67
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human COL4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human COL4 in samples. No significant cross-reactivity or interference between Human COL4 and analogues was observed.
